Terragene Bionova® BT20 Self-Contained Biological Indicators have been designed for monitoring of vacuum assisted and gravity air displacement Steam sterilization cycles at 121 to 135°C. If the sterilization process was not effective, the indicator medium will turn from purple to yellow after incubation between 55 to 62°C, thus indicating the presence of living Geobacillus stearothermophilus spores. If the sterilization process was effective, the indicator medium will remain purple after incubation. The final readout should be performed at 24 hours of incubation between 55 to 62°C.
- For steam sterilization processes
- Geobacillus Stearothermophilus (ATCC 7953) 105 or 106 spores per vial
- Purple growth indicator medium contained within the glass ampoule
- Results in 24 hours